ABSTRACT
OBJECTIVE:To optimize the clarification and purification technology of Perillae folium extract. METHODS:The effects of 3 clarification and purification methods as chitosan flocculation clarification,ZTC 1+1-Ⅱflocculation clarification,water precipitation on retention rate of total flavonoids and removal rate of solid of Perillae folium extract were compared to screen suit-able clarification and purification technology. With the retention rate of total flavonoids and removal rate of solid as comprehensive evaluation index,single factor and orthogonal test were designed to investigate the optimal value of concentration proportion,the amount of the flocculant,flocculation temperature and whisking speed in optimal clarification and purification method. RESULTS:Among 3 methods,the chitosan flocculation clarification was the best with concentration proportion of 1∶4,chitosan of 1.0 g/L, flocculation temperature at 60 ℃,whisking speed of 100 r/min,whisking time of 4 min,standing time of 12 h. Under the condi-tion of optimal processing,the retention rate of total flavonoids was (85.1 ± 0.75)%,and the removal rate of solid was (24.6 ± 1.33)%(n=5). CONCLUSIONS:Chitosan flocculation can be used to effectively remove the impurity of Perillae folium extract, and optimized clarification and purification technology is stable and feasible.
ABSTRACT
OBJECTIVE:To optimize the formulation and technology of poly(lactide-co-glycolide)(PLGA)microspheres con-taining total alkaloids of Panzeria alaschanica,and to prepare microspheres and conduct quality investigation. METHODS:PLGA microspheres containing total alkaloids of P. alaschanica (PTPM) was prepared by double emulsion-solvent evaporation method. The formulation of microspheres was optimized by L9(34) orthogonal design using mass concentration of PLGA,PVA concentra-tion,ratio of water phase to oil phase as factor,drug-loading amount,encapsulation efficiency,yield as index. The morphology, particle size and drug release of microspheres were all investigated. RESULTS:The optimal formulation was as follows as the mass concentration of PLGA 200 mg/ml,the concentration of PVA 2%,and the water phase-oil phase ratio 1:5. In validation test,aver-age encapsulation efficiency was(83.2±2.4)%,average drug-loading amount(4.16±0.17)%,average yield(86.7±3.6)%,and comprehensive score (95.7 ± 4.4)%;all RSDs were lower than 5.0%(n=3). Prepared microspheres were spherical with smooth surface and uniform particle size. The average particle size was(22.3±2.4)μm and accumulative release rate of 24 h was(82.3± 3.5)%,which conformed to first-order release model(r=0.972 4). CONCLUSIONS:Optimized technology is stable. Prepared mi-crospheres show good sustained-release property,and fit to quality requirements.
ABSTRACT
OBJRCTIVE:To study the feasibility and the preparation of bone morphogenetic protein-2(BMP 2 )carrying gel microspheres by dextran-glycidyl methacrylate(DEX-GMA)and to make an initial study on the drug-loading and drug releasing function of gel.METHODS:The orthogonal test was conducted with the reaction temperature,the addition of DEX-GMA and Span-80,the stirring speed and so on as investigation factors,the best preparation technics of BMP 2 -DEX-GMA gel microspheres was optimized and the finished products of which were given an initial determination.RE-SULTS:The BMP 2 -DEX-GMA gel microspheres preparation could be made by suspension polymerization,the optimized preparation technics including the reaction temperature was30℃,the addition of DEX-GMA and Span-80were0.6%and0.06%respectively,the stirring speed was300r/min,the BMP 2 -DEX-GMA gel microspheres from the above formulation take good shapes with the particle diameter at20?m～50?m,the envelopment ratio was(78.52?4.34)%,the quantity of drug-loading was(10.68?1.34)%,and the swelling ratio was85.9%,which has a good stability and redispersibility.CON-CLUSION:The preparation technics of gel microspheres drug-loading system is simple and the loading dosage is high,which can be used as a bioactive drugs carrier.